NGS Application
PSI-Seq (Pseudouridine Site Identification)
Period Of Training
7 days
Training Schedule
2 Hours Daily (One To One Training) Monday to Friday
Training Slots
Our working Time is 9:00 AM to 6:00 PM Indian Time
Available
slots - 9:00 AM to 11:00 AM / 11:00 AM to 1:00 PM / 2:00 PM to 4:00 PM / 4:00 PM to 6:00
PM
Training Mode
Online/Offline
Online(For online training candidate have to
install Skype and AnyDesk)(it is same like offline)
Offline(For offline
training candidate have to come to head office Pune and bring their own laptop)
All our training is one to one and 100% focus with individual candidate which provides 100% learning.
Sequencing Platform
Illumina / Ion Torrent
if other platform pacbio or nanopore or
any other sequencing platform kindly write email to us.
Raw data
Training will be based on same content provided as well as demo raw data from NCBI SRA will be taken.
Kindly note if custom requirement either to change the content or tools or raw data from candidate to be included during training
in that case it will come under custom training and we request to send us email details accordingly.
Also candidate can learn with their own raw data as well as can also make all figures and tables as per the publication reports which will help to directly make reports with our expert team.
Kindly note only custom based training will provide this feature and need to be discussed before registration.
Training Starting Date
Registration - Open
Training can be started on any date as per the candidate convenience
as its one to one training as well as its 100% interactive.
Module-NGS
PSI-Seq Data Analysis
-Introduction to Bioinformatics and Overview of NGS & detailed understanding
-Linux understanding and Installation of Linux and NGS tools
- Data Retrieval (NCBI SRA) & Data types
- Identification of Pseudouridine sites using CMCT
- Read Quality Check (FastQC & Cutadapt)
- Alignment of reads using reference Genome (Bowtie2)
- Visualization of mapped reads (IGV)
- Generate metrics at single nucleotide positions of Reference Genome
(bam-readcount)
- Basics on R programming
- Pseudouridine site identification by calculating the "pseudouridylation scores"
- Downstream analysis of the identified pseudo-uridine sites(Annotation,GO and Pathway
enrichment analysis)
- Generating Advanced Plots(h-plot, scatter-plot,heatmap) to represent
Pseudo-uridylation sites(using R)
- Channelling the analysis towards the regulation effect of the pseudo uridines in the
mRNA- Combining RNA-Seq with PSI-Seq analysis
- Gene Expression Quantification(ht-seq)
- Downstream analysis of differentially expressed genes(Annotation,GO and Pathway
enrichment analysis)
- Pathway Network analysis using stringDB(PPI) & Cytoscapes
Eligibility
Anyone who is interested can apply (Any Life sciences graduate/postgraduate/PhD).
Industrial Exposure
100% based on Industrial exposure/Research
Certificate
All the participants will receive certificates on successful
completion of training
Support
- live recording during training as well as training manual will be
provided
- Additional 2hrs of support after completion of training
- server
access if the candidate laptop/desktop will not support for analysis
Payment Options
Paypal/Wire Transfer/cheque/Gpay/PhonePay/Online Gateway
Razorpay(National and International)/Any UPI Payment