Metatranscriptomics

Slots

Our working Time is 9:00 AM to 6:00 PM Indian Time Available slots - 9:00 AM to 11:00 AM / 11:00 AM to 1:00 PM / 2:00 PM to 4:00 PM / 4:00 PM to 6:00 PM
For training slots after 6 PM or before 9 am as well as weekends training kindly mention during registration accordingly it will be scheduled.

Mode

👉 For online training candidate have to install ZOOM (with remote control on candidate system which makes 100% interactive)
👉 For offline training candidate need to come to head office pune, India
👉 Run time video recording candidate can make as well as pdf manual will be provided for future reference.
👉 All our training is 100% practical and 100% industrial and 100% interactive which provides same as offline learning.
👉 For doubt clear there will be extra support will be provided based on the requirement.
👉 Certificate will be provided

📘 Introduction to Bioinformatics
- Overview of bioinformatics and its applications
- Key concepts in computational biology
- Role of bioinformatics in genomics, transcriptomics, and proteomics

📘 Understanding NGS and Genomics Bioinformatics
- Basics of Next-Generation Sequencing (NGS)
- Types of NGS data (RNA-seq, WGS, WES)
- Overview of NGS data formats: FASTQ, BAM, VCF
- Introduction to pipelines and tools for NGS data analysis

📘 Databases & Data Retrieval (NCBI and UCSC)
- Learning how to retrieve biologically correct data
- Performing complete batch retrieval (e.g., whole exome)
- NCBI: understanding gene-level data retrieval
- UCSC: handling large-scale data retrieval
- UCSC Genome Browser and Table Browser usage
- Batch Coordinate Retrieval and Genomic Data downloads
- GFF/GTF gene annotation formats and how to retrieve them
- Using BLAT for sequence-based search and alignment

📘 Gene Prediction and Functional Annotation
- Gene prediction approaches and tools
- Functional annotation using Gene Ontology (GO)
- Pathway analysis using KEGG, Reactome
- Interpreting gene sets and biological relevance

📘 Standalone/Offline BLAST for Large-Scale Genomic Data
- Installing and setting up standalone BLAST
- Running local BLAST for batch sequence alignment
- Applications in genome-wide homology searches
- Custom BLAST databases and performance optimization

📘 PCR Primer Designing and Specificity Check
- Designing accurate primers for PCR amplification
- Tools: Primer3, NCBI Primer-BLAST
- Checking primer specificity using genome-wide BLAST
- Avoiding non-target amplification through design best practices

📘 Understanding Python Programming
- Introduction to Python for bioinformatics
- Scripting basics: variables, loops, functions
- Libraries like Biopython, pandas for biological data handling
- Automating genomic workflows with Python scripts

📘 Introduction to NGS Metatranscriptome
- Understanding NGS and Its Applications
- Types of sequencing data generated
- Understanding FASTQ files and sequencing quality scores

📘 Linux Basics & Environment Setup
- Linux Command Line Basics
- Installing WGS Metagenome Tools
- Using Conda and Shell Scripting

📘 Data Retrieval & Formats
- Fetching data from NCBI SRA using SRA Toolkit
- Understanding different file formats

📘 Introduction to R/Bioconductor
- Installing packages with CRAN and Bioconductor
- Data types and standardized data container
- Data manipulation

📘 Detailed Metatranscriptome Data Analysis Pipeline


1. - Assembly:
   Assembling short reads into longer contigs to reconstruct genomic fragments
2. - RNA Prediction and Classification:
   Detecting ribosomal RNA and other non-coding RNAs from assembled sequences and classifying them into known RNA families
3. - ORF (CDS) Prediction:
   Predicting open reading frames (ORFs) or coding sequences (CDS) using tools like Prodigal/prokka to identify potential genes
4. - Homology Searching Against Taxonomic and Functional Databases:
   Matching predicted genes against reference databases (e.g., NCBI NR, COG, KEGG) to infer function and taxonomy
5. - HMMER Searching Against Pfam Database:
   Identifying protein domains using HMMER against the Pfam database to support functional annotation
6. - Taxonomic Assignment of Genes:
   Assigning taxonomy to each gene based on homology search results
7. - Functional Assignment of Genes:
   Linking predicted genes to functional categories like metabolic pathways or gene families using KEGG, COG etc.
8. - Blastx on Parts of the Contigs with No Gene Prediction or Hits:
   Running Blastx on contig regions that lack gene predictions or functional hits to discover missed genes
9. - Taxonomic Assignment of Contigs and Disparity Checks:
   Assigning taxonomy at the contig level and verifying consistency among genes within each contig to avoid chimeras
10. - Coverage and Abundance Estimation for Genes and Contigs:
    Calculating how abundant each gene or contig is by mapping sequencing reads back to the assembly
11. - Estimation of Taxa Abundances:
    Quantifying the abundance of microbial taxa across samples based on read mappings and gene assignments
12. - Estimation of Function Abundances:
    Estimating the abundance of biological functions or pathways in the community using annotated gene data
13. - Merging of Previous Results to Obtain the ORF Table:
    Integrating taxonomic, functional, and abundance data into a single table summarizing all ORFs
14. - Merging of Previous Results to Obtain the Merged Data:
    Creating an abundance matrix summarizing all gene content and count of number of reads
15. - Generation of Tables with Aggregated Taxonomic and Functional Profiles:
    Producing overview tables that summarize taxonomic composition and functional potential across samples
16. - Visualization:
    Creating rich visual outputs including PCA plots, rarefaction curves, stacked bar charts, heatmaps, Krona charts, volcanoplot, GO and pathway enrichment, and network analysis to interpret the data effectively

Preparation

- Metatranscriptomics : https://en.wikipedia.org/wiki/Metatranscriptomics
- NCBI : https://pubmed.ncbi.nlm.nih.gov/39029911/

Instructor

Industry Experienced

Target Audiance

This course is designed for graduate students, postdoctoral researchers, and professionals working in the fields of conservation biology, evolutionary genomics, and population genetics or any life sciences who are interested in applying genomic tools to real-world conservation challenges.

Contact

Please write us at info@arraygen.com or call or whatsapp us on mobile +91-9673625446 if you need any clarification or for any custom training based on candidate reference paper or candidate own content/tools.