CRISPR/Cas9

CRISPR-Cas9 is a powerful gene-editing tool that allows scientists to precisely modify DNA in living cells. It works by using a guide RNA to direct the Cas9 enzyme to a specific DNA sequence, where it makes a targeted cut. This technology is revolutionizing medicine, agriculture, and biotechnology by enabling faster, cheaper, and more accurate genetic changes than ever before.

ArrayGen specialize in sgRNA (single guide RNA) design and CRISPR-Cas9 data analysis, helping researchers ensure accurate and effective gene editing.

Custom sgRNA design for target-specific genome editing
Off-target prediction and risk assessment
CRISPR mutation analysis and result interpretation
Learners will gain a clear understanding of how CRISPR-Cas9 utilizes sgRNA and the Cas9 enzyme to perform precise gene editing. They will learn the importance of designing effective sgRNAs for targeting specific DNA sequences and how to predict and assess potential off-target effects. Additionally, learners will develop skills to analyze mutation data to evaluate the success of CRISPR experiments and will become familiar with using ArrayGen’s specialized tools to enhance the accuracy and efficiency of gene editing projects.

👉 Enroll now and take the first step toward a future in data-driven biology and beyond.

## 10% Discount if you register before 15th October, 2025. Hurry up!!

CRISPR/Cas9 Training Information

Course

CRISPR/Cas9

Duration

Online - 15 Days Training [ 2 Hours Daily [ Monday To Friday ] ]

Slots

Our working Time is 9:00 AM to 6:00 PM Indian Time Available slots - 9:00 AM to 11:00 AM / 11:00 AM to 1:00 PM / 2:00 PM to 4:00 PM / 4:00 PM to 6:00 PM
For training slots after 6 PM or before 9 am as well as weekends training kindly mention during registration accordingly it will be scheduled.

Mode

👉 For online training candidate have to install ZOOM (with remote control on candidate system which makes 100% interactive)
👉 Run time video recording candidate can make as well as pdf manual will be provided for future reference.
👉 All our training is 100% practical and 100% industrial and 100% interactive which provides same as offline learning.
👉 For doubt clear there will be extra support will be provided based on the requirement.
👉 Certificate will be provided
Training Fees

Raw Data

Candidate can provide their own target sequence as well as CRISPR edited sequencing data for analysis(only one sample data will be included during training). Publication standards figures and tables will be generated.

Platform

Illumina/Nanopore/Pacbio/PCR Based Amplification (any one platform will be included as per candidate selection)

Organism

Plant/Bacteria/Animalia/Eukaryotic/Prokaryotic (any orginism)

Module - 1

Advanced Bioinformatics(Target sequence selection)

Topics

    📘 Introduction to Bioinformatics
    - Overview of bioinformatics and its applications
    - Key concepts in computational biology
    - Role of bioinformatics in genomics, transcriptomics, and proteomics

    📘 Understanding NGS and Genomics Bioinformatics
    - Basics of Next-Generation Sequencing (NGS)
    - Types of NGS data (RNA-seq, WGS, WES)
    - Overview of NGS data formats: FASTQ, BAM, VCF
    - Introduction to pipelines and tools for NGS data analysis

    📘 Databases & Data Retrieval (NCBI and UCSC)
    - Learning how to retrieve biologically correct data
    - Performing complete batch retrieval (e.g., whole exome)
    - NCBI: understanding gene-level data retrieval
    - UCSC: handling large-scale data retrieval
    - UCSC Genome Browser and Table Browser usage
    - Batch Coordinate Retrieval and Genomic Data downloads
    - GFF/GTF gene annotation formats and how to retrieve them
    - Using BLAT for sequence-based search and alignment

    📘 Gene Prediction and Functional Annotation
    - Gene prediction approaches and tools
    - Functional annotation using Gene Ontology (GO)
    - Pathway analysis using KEGG, Reactome
    - Interpreting gene sets and biological relevance

    📘 Standalone/Offline BLAST for Large-Scale Genomic Data
    - Installing and setting up standalone BLAST
    - Running local BLAST for batch sequence alignment
    - Applications in genome-wide homology searches
    - Custom BLAST databases and performance optimization

    📘 PCR Primer Designing and Specificity Check
    - Designing accurate primers for PCR amplification
    - Tools: Primer3, NCBI Primer-BLAST
    - Checking primer specificity using genome-wide BLAST
    - Avoiding non-target amplification through design best practices

    📘 Understanding Python Programming
    - Introduction to Python for bioinformatics
    - Scripting basics: variables, loops, functions
    - Libraries like Biopython, pandas for biological data handling
    - Automating genomic workflows with Python scripts

Module - 2

CRISPR/Cas9(sgRNA Designing & Data analysis)

Topics

    📘 Introduction to CRISPR
    - Overview of CRISPR technology and its applications

    📘 sgRNA Designing
    - Understanding single guide RNA (sgRNA)
    - Practical strategies for sgRNA design

    📘 Next-Generation Sequencing (NGS)
    - Introduction to NGS technology and data types
    - Data retrieval from NCBI SRA
    - Understanding sequencing data formats

    📘 Data Preprocessing
    - Read quality control
    - Cleaning raw sequencing data

    📘 Read Alignment & Mapping
    - Aligning reads to reference genome
    - Mapping output analysis (SAM/BAM files)

    📘 Variant Analysis
    - Variant detection from sequencing data
    - Visualization of variants (IGV)
    - Variant annotation and effect prediction

    📘 Mutation Analysis in CRISPR-Edited Genomes
    - Comprehensive mutation analysis
    - Interpreting mutation data for editing outcomes

    📘 NCBI Submission Guideline

    NOTE: CRISPR training is only based on dry lab(no wet lab). The course includes sgRNA designing and CRISPR-edited data analysis & interpretation.

Preparation

- CRISPR: https://en.wikipedia.org/wiki/CRISPR
- NCBI: https://www.ncbi.nlm.nih.gov/
- UCSC : https://genome.ucsc.edu/

Instructor

Industry Experienced

Target Audiance

This course is designed for graduate students, postdoctoral researchers, and professionals working in the fields of conservation biology, evolutionary genomics, and population genetics or any life sciences who are interested in applying genomic tools to real-world conservation challenges.

Contact

Please write us at info@arraygen.com or call or whatsapp us on mobile +91-9673625446 if you need any clarification or for any custom training based on candidate reference paper or candidate own content/tools.