RAD-Seq

At ArrayGen Technologies, we offer comprehensive solutions and training for RAD-seq (Restriction site Associated DNA Sequencing) data analysis a powerful next-generation sequencing (NGS) approach for genome-wide SNP discovery and genotyping.
RAD-seq is widely used in population genomics, evolutionary studies, and genetic diversity assessment, especially in non-model organisms where reference genomes are limited or absent. Our customized workflow includes both reference-based and de novo pipelines.
Whether you're conducting research in plant and animal breeding, ecological genetics, or evolutionary biology, ArrayGens expertise ensures high-quality, reproducible results from raw reads to population structure analysis.

By completing the RADseq pipeline, learners gain practical skills in SNP discovery and genotyping, preparing them for academic research, clinical genomics, or biotech industry roles.

👉 Enroll now and take the first step toward a future in data-driven biology and beyond.

## 10% Discount if you register before 15th October, 2025. Hurry up!!

RNASeq [R]
RNASeq [D]
scRNA-Seq
lncRNA-Seq
miRNA-Seq
PSI-Seq
ChipSeq
ATAC-Seq
scATA-Seq
Spatial Transcriptome
DNASeq
MethylSeq
Whole Genome de novo Assembly
Targeted metagenomics
Whole Genome Metagenomics
Metatranscriptomics
RAD-Seq
SNAP-ChIP

7 Days Training
15 Days Training

Course Information
Course	RAD-Seq Data Analysis
Duration	7 Days Training [ 2 Hours Daily [ Monday To Friday ] ]
Slots	Our working Time is 9:00 AM to 6:00 PM Indian Time Available slots - 9:00 AM to 11:00 AM / 11:00 AM to 1:00 PM / 2:00 PM to 4:00 PM / 4:00 PM to 6:00 PM For training slots after 6 PM or before 9 am as well as weekends training kindly mention during registration accordingly it will be scheduled.
Mode	Online (one to one individual Focused Training) 👉 For online training candidate have to install ZOOM (with remote control on candidate system which makes 100% interactive) 👉 Run time video recording candidate can make as well as pdf manual will be provided for future reference. 👉 All our training is 100% practical and 100% industrial and 100% interactive which provides same as offline learning. 👉 For doubt clear there will be extra support will be provided based on the requirement. 👉 Certificate will be provided
Sequencing Platform	Illumina
Raw data	Candidate can include maximum 4 datasets of their own during training. Publication standards figures and tables will be generated.
Training Fees
Support	Candidate can also discuss and possible implementation with their own genomic data or any data too during the course.
Module-NGS RAD-Seq Data Analysis	📘 RAD-Seq Basics - Understanding NGS and Application RAD-Seq and understanding different data formats 📘 Linux and Tool Installation - Linux installation and NGS tools installation 📘 Quality Control - Quality control of raw reads: Check the quality of sequencing reads and detect issues like adapter contamination or low-quality bases - Demultiplexing samples by barcode: Separate sequencing reads into individual samples using sample-specific barcodes with process_radtags - Trim adapters and low-quality bases: Remove adapter sequences and trim poor-quality ends from reads using Trimmomatic 📘 Read Alignment and Assembly - Align reads or assemble loci de novo: Raw reads were mapped based on reference or denovo based 📘 SNP Calling and Filtering - Call SNPs and genotype samples: Identify SNPs and assign genotypes using Stacks - Filter SNPs by quality and coverage: SNPs were filtered based on coverage depth, minor allele frequency, and missing data 📘 Population Genomics Analysis - Population genomics analyses: Post analysis such as PCA, genetic structure, and diversity studies 📘 Variation Visualization - Variation Vizualization: Variation vizualization using IGV browser
Preparation	- RAD-Seq : https://en.wikipedia.org/wiki/Restriction_site_associated_DNA_markers - NCBI : https://pmc.ncbi.nlm.nih.gov/articles/PMC3080771/
Instructor	Industry Experienced
Target Audiance	This course is designed for graduate students, postdoctoral researchers, and professionals working in the fields of conservation biology, evolutionary genomics, and population genetics or any life sciences who are interested in applying genomic tools to real-world conservation challenges.
Contact	Please write us at info@arraygen.com or call or whatsapp us on mobile +91-9673625446 if you need any clarification or for offline training or for any custom training based on candidate reference paper or candidate own content/tools.

Course Information
Course	RAD-Seq Data Analysis
Duration	15 Days Training [ 2 Hours Daily [ Monday To Friday ] ]
Slots	Our working Time is 9:00 AM to 6:00 PM Indian Time Available slots - 9:00 AM to 11:00 AM / 11:00 AM to 1:00 PM / 2:00 PM to 4:00 PM / 4:00 PM to 6:00 PM For training slots after 6 PM or before 9 am as well as weekends training kindly mention during registration accordingly it will be scheduled.
Mode	Online (one to one individual Focused Training) 👉 For online training candidate have to install ZOOM (with remote control on candidate system which makes 100% interactive) 👉 Run time video recording candidate can make as well as pdf manual will be provided for future reference. 👉 All our training is 100% practical and 100% industrial and 100% interactive which provides same as offline learning. 👉 For doubt clear there will be extra support will be provided based on the requirement. 👉 Certificate will be provided
Sequencing Platform	Illumina
Raw data	Candidate can include maximum 4 datasets of their own during training. Publication standards figures and tables will be generated.
Training Fees
Support	Candidate can also discuss and possible implementation with their own genomic data or any data too during the course.
Module-I	Advanced Bioinformatics & basic programming
Topics	📘 Introduction to Bioinformatics - Overview of bioinformatics and its applications - Key concepts in computational biology - Role of bioinformatics in genomics, transcriptomics, and proteomics 📘 Understanding NGS and Genomics Bioinformatics - Basics of Next-Generation Sequencing (NGS) - Types of NGS data (RNA-seq, WGS, WES) - Overview of NGS data formats: FASTQ, BAM, VCF - Introduction to pipelines and tools for NGS data analysis 📘 Databases & Data Retrieval (NCBI and UCSC) - Learning how to retrieve biologically correct data - Performing complete batch retrieval (e.g., whole exome) - NCBI: understanding gene-level data retrieval - UCSC: handling large-scale data retrieval - UCSC Genome Browser and Table Browser usage - Batch Coordinate Retrieval and Genomic Data downloads - GFF/GTF gene annotation formats and how to retrieve them - Using BLAT for sequence-based search and alignment 📘 Gene Prediction and Functional Annotation - Gene prediction approaches and tools - Functional annotation using Gene Ontology (GO) - Pathway analysis using KEGG, Reactome - Interpreting gene sets and biological relevance 📘 Standalone/Offline BLAST for Large-Scale Genomic Data - Installing and setting up standalone BLAST - Running local BLAST for batch sequence alignment - Applications in genome-wide homology searches - Custom BLAST databases and performance optimization 📘 PCR Primer Designing and Specificity Check - Designing accurate primers for PCR amplification - Tools: Primer3, NCBI Primer-BLAST - Checking primer specificity using genome-wide BLAST - Avoiding non-target amplification through design best practices 📘 Understanding Python Programming - Introduction to Python for bioinformatics - Scripting basics: variables, loops, functions - Libraries like Biopython, pandas for biological data handling - Automating genomic workflows with Python scripts
AND
Module-II	Next Generation Sequencing (NGS) - RAD-Seq Data Analysis
Topics	📘 RAD-Seq Basics - Understanding NGS and Application RAD-Seq and understanding different data formats 📘 Linux and Tool Installation - Linux installation and NGS tools installation 📘 Quality Control - Quality control of raw reads: Check the quality of sequencing reads and detect issues like adapter contamination or low-quality bases - Demultiplexing samples by barcode: Separate sequencing reads into individual samples using sample-specific barcodes with process_radtags - Trim adapters and low-quality bases: Remove adapter sequences and trim poor-quality ends from reads using Trimmomatic 📘 Read Alignment and Assembly - Align reads or assemble loci de novo: Raw reads were mapped based on reference or denovo based 📘 SNP Calling and Filtering - Call SNPs and genotype samples: Identify SNPs and assign genotypes using Stacks - Filter SNPs by quality and coverage: SNPs were filtered based on coverage depth, minor allele frequency, and missing data 📘 Population Genomics Analysis - Population genomics analyses: Post analysis such as PCA, genetic structure, and diversity studies 📘 Variation Visualization - Variation Vizualization: Variation vizualization using IGV browser
Preparation	- RAD-Seq : https://en.wikipedia.org/wiki/Restriction_site_associated_DNA_markers - NCBI : https://pmc.ncbi.nlm.nih.gov/articles/PMC3080771/
Instructor	Industry Experienced
Target Audiance	This course is designed for graduate students, postdoctoral researchers, and professionals working in the fields of conservation biology, evolutionary genomics, and population genetics or any life sciences who are interested in applying genomic tools to real-world conservation challenges.
Contact	Please write us at info@arraygen.com or call or whatsapp us on mobile +91-9673625446 if you need any clarification or for offline training or for any custom training based on candidate reference paper or candidate own content/tools.